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In one embodiment, the method comprises introducing into the prokaryotic organism an expressible nucleic acid encoding a putatively essential gene for proliferation or pathogenicity under the control of a promoter and at least one tet operator, and an expression vector comprising a nucleotide sequence encoding a modified tetracycline repressor, wherein said modified tetracycline repressor binds to a tetracycline operator sequence in a prokaryotic organism with a greater affinity in the presence of tetracycline or a tetracycline analog than in the absence of tetracycline or a tetracycline analog?

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